Lipid-based transfection is a powerful and widely adopted approach for delivering nucleic acids into mammalian cells. Yet many adherent cell linesremain notoriously difficult to transfect, limiting efficiency and reproducibility.
EZ-Fect™ overcomes this challenge. Built on a next-generation lipid platform, EZ-Fect™ delivers higher expression, lower cytotoxicity, and consistent performance—even in cells where conventional reagents fall short.
How EZ-Fect™ Works
EZ-Fect™ employs a three-step, precision-engineered formulation to optimize every stage of nucleic acid delivery:
Tube 1 — Optimized Lipoplex Formation A proprietary lipid blend forms uniformly sized lipoplexes for efficient plasma membrane fusion.
Tube 2 — Nuclear Transfer Enhancement A unique enhancer promotes transport of nucleic acids across the nuclear envelope.
Tube 3 — Accelerated Nuclear Delivery A specialized cocktail facilitates rapid trafficking from cytoplasm to nucleus.
Together, these synergistic components enable robust, reproducible transfection in historically difficult-to-transfect adherent cell types—outperforming leading lipid-based alternatives.
Blow are representative transfection results across 3 historically highly challenging cell lines (L929, A431, BJ) along with a positive control cell line A549: